- D. PCR相關產品
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- One-Step RT-PCR Kit III, RP01-III
One-Step RT-PCR Kit III, RP01-III
The One-Step RT-PCR Kit llI provides one-tube, two-enzyme system for the reverse transcription and PCR amplification of a specific target RNA from either total RNA or mRNA. The system uses SuperSAMscript III reverse transcriptase for first-strand cDNA synthesis and GenHot DNA polymerase for PCR amplification. SuperSAMscript III RTase is a genetically engineered version of M-MLV RTase which lacks 3’ to 5’ exonucleolytic proofreading function and has a no RNase H activity as compared to AMV RTase, that is thermo stable (amplified DNA in 37~55 ℃ ) and is useful for full-length cDNA amplification(~15kb). GenHot Taq DNA polymerase in the kit is modified with specific monoclonal antibody and can eliminate amplification artifacts such as primer-dimer formation and mis-priming during pre-amplification stage.
Size: 50 rxn; 250 rxn (50 μl/rxn)